ANO1 anticorps

N° du produit ABIN6252316

L’anticorps Souris Monoclonal anti-ANO1 a été validé pour IHC, IF et FACS. Il convient pour détecter ANO1 dans des échantillons de Humain.

Aperçu rapide pour ANO1 anticorps (ABIN6252316)

Antigène: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ANO1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM580

Détail du produit anti-ANO1 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human DOG1 protein was used as the immunogen for this ANO1 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the ANO1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur ANO1

Antigène: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Autre désignation: ANO1

Sujet: Expression of DOG-1/ANO1/TMEM16A protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. ANO1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG-1/ANO1 and c-kit in GISTs is nearly identical: 94.4 % vs. 94.7 %.

ID gène: 55107

UniProt: Q5XXA6